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• Buffered salt solutions are made to a physiological pH and salt concentration
• Most commonly include sodium, potassium, calcium, magnesium and chloride
• They provide the cells with water and inorganic ions, while maintaining a physiological pH and osmotic pressure

Dulbecco’s Phosphate Buffered Saline

• DBSS is a buffer solution, used to maintain cell culture media in the physiological pH range of 7.0 to 7.6
• Can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time

Hanks Balanced Salt Solution

• HBSS is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions